Observing and tracking single small ribosomal subunits in vivo.

Methods(2019)

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摘要
•Indirect labeling of RNA particles provides essentially “native labeling”.•The majority of the small ribosomal subunits is immobilized inside nucleoli.•1/3 of the small ribosomal subunits diffuses freely in the nucleoplasm.•1/3 of the small ribosomal subunits shows unexpected immobilizations in nucleoplasm.
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关键词
Ribosomal biogenesis,Small ribosomal subunit,Snap-tag,Single particle tracking,RNA trafficking
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