The garlic allelochemical DADS influences cucumber root growth involved in regulating hormone levels and modulating cell cycling.

Most allelochemicals are phytotoxic to the receiver plants and may influence the cell micro- and ultrastructure, cell division, phytohormone levels, and ultimately growth. In order to understand the allelopathic potential of garlic, the effects of its main bioactive allelochemical diallyl disulfide (DADS), experiments were carried out to observe the seed germination, root growth, and developmental responses in cucumber seedlings treated with various concentrations of DADS. The obtained data suggested active influence of DADS on cucumber root growth and development. Significant responses were observed in early root growth and elongation, mitotic cell division and elongation, and root architecture modulation. The effect, however, was dose dependent, and lower concentrations of DADS proved to be promotional whereas higher concentrations of DADS inhibited cucumber root growth and development. Relative root elongation (RRE) revealed that DADS could increase growth of cucumber roots in the early developmental days. Moreover, DADS application significantly influenced mitosis-related gene expression. Observed genes CYCA and CDKB were initially downregulated in the first 24 h but significantly upregulated after 48 h, while gene CDKA was upregulated in the first 24 h. Similarly, DADS application significantly altered primary plant hormones, such as IAA, ABA, GA3, and ZR, in the cucumber roots. Taken together, low concentrations of DADS treatment could promote cucumber root growth and induce main root elongation by upregulating CDKA and CDKB gene expression and regulating hormone balance in root. The current findings offer insight into the allelopathic potential of garlic allelochemical DADS and can be considered vital for establishing plant allelopathic studies.