Protocol for Generating Infectious RNA Viromes from Complex Biological Samples.

Sonia Monteil-Bouchard,Sarah Temmam,Christelle Desnues

HUMAN VIROME: METHODS AND PROTOCOLS（2018）

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摘要

This chapter proposes a simple, standardized protocol for generating RNA viromes from complex host-associated biological samples of various origins. Compared to other existing protocols to generate RNA viromes, this protocol preserves the infectivity of viral particles and allows for downstream applications such as viral characterization and isolation tests.

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关键词

Virome, Arthropods, Metagenomics, RNA viruses, Sucrose density gradient, Viral zoonosis

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