Assessing Hepatitis B immunity using dried blood spot samples from HIV individuals.
JOURNAL OF MEDICAL VIROLOGY(2018)
摘要
This study aims to evaluate the utility of an optimized enzyme immunoassay (EIA) to detect and quantify antibodies against hepatitis B surface antibody (anti-HBs) in dried blood spots (DBSs) within the context of human immunodeficiency virus (HIV) status. Serum and DBS samples were obtained from 56 HIV+ and 99 HIV- patients and subjected to EIA for the detection of anti-HBs, where sample volume and cut off value were modified for DBS testing. Sensitivities of anti-HBs detection in DBS were 79.8% and 76.8% in HIV- and HIV+ subjects, respectively. Concordant results for anti-HBs in serum and DBS presented high mean CD8 cell counts, HIV viral load and optical density (OD) values of anti-HBs. Anti-HBs titers were significantly higher in serum, whether or not anti-HBs titers were detected in DBS. It was possible to detect anti-HBs in DBS as low as 17.4 and 27.3IU/mL among HIV+ and HIV- subjects, respectively. In conclusion, DBS can be used to detect and quantify anti-HBs in HIV-infected individuals, which could increase access to diagnosis and vaccination. Highlights Evaluation of anti-HBs detection in DBS according to HIV status.Similar performance of anti-HBs detection from DBS independent of HIV status.Detection of anti-HBs in concentration up to 17 IU / mL in DBS.DBS could be a potential tool to increase identification of hepatitis B immunity.
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关键词
diagnosis,dried blood spot,hepatitis B,immunodeficiency virus,immunity
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