Comparison of the antioxidant activity of catalase immobilized on gold nanoparticles via specific and non-specific adsorption.

Ewa Czechowska,Katarzyna Ranoszek-Soliwoda,Emilia Tomaszewska,Agnieszka Pudlarz,Grzegorz Celichowski, Dorota Gralak-Zwolenik,Janusz Szemraj,Jaroslaw Grobelny

Colloids and Surfaces B: Biointerfaces（2018）

引用 26|浏览7

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摘要

•Catalase successfully immobilized via specific and non-specific methods on AuNPs.•The surface coverage of NPs with catalase determined via gel electrophoresis.•Enzymatic activity CAT-AuNPs bjoconjugates determined.•Bioconjugates containing biologically active catalase on AuNPs prepared.•The two methods of protein immobilization on NPs compared.

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关键词

Catalase-modified nanoparticles,Enzyme immobilization,Catalase,Gel electrophoresis

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