Simultaneous quantification of EVT201, a novel partial positive allosteric GABA A receptor modulator, and its two metabolites in human plasma by UHPLC/MS/MS.

•EVT201 is a novel partial GABAA receptor agonist to treat insomnia.•A sensitive and accurate UHPLC-MS/MS method was developed to determine EVT201 and its two metabolites in human plasma.•The method was successfully applied to the sample analysis of a Phase I clinical trial.