Analytical validation of viral CNS Flow Chip kit for detection of acute meningitis and encephalitis.

•A new assay for the diagnosis of viral neurological infections is described.•Based on multiplex PCR followed by automatic flow-through hybridization.•Eight viral targets are included in the panel.•Toscana virus, endemic in Mediterranean countries is included in the panel.•Overall sensitivity and specificity: 95.9% and 99.9%, respectively.