RNA-Seq and metabolic flux analysis of Tetraselmis sp. M8 during nitrogen starvation reveals a two-stage lipid accumulation mechanism.

David K Y Lim,Holger Schuhmann,Skye R Thomas-Hall,Kenneth C K Chan,Taylor J Wass,Felipe Aguilera,T Catalina Adarme-Vega,Cristiana G O Dal'Molin, Glen J Thorpe,Jacqueline Batley,David Edwards,Peer M Schenk

Bioresource Technology（2017）

引用 31|浏览2

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摘要

•Tetraselmis RNA-Seq was performed at early lipid accumulation after N deprivation.•qRT-PCR analyses revealed distinct phases for lipid biosynthesis and catabolism.•Gene expression data was combined with metabolic reconstruction modeling.•Tetraselmis shifts from reduced lipid consumption to active lipid production.•This process appears independent from DGAT expression.

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关键词

Beta-oxidation,Metabolic engineering,Microalgae,RNA-Seq,TAG biosynthesis

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