Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A.

Keita Omura,Yuichiro Aiba,Hiroki Onoda,Joshua Kyle Stanfield,Shinya Ariyasu,Hiroshi Sugimoto,Yoshitsugu Shiro,Osami Shoji,Yoshihito Watanabe

CHEMICAL COMMUNICATIONS（2018）

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摘要

Haem substitution is an effective approach to tweak the function of haemoproteins. Herein, we report a facile haem substitution method for self-sufficient cytochrome P450BM3 (CYP102A1) from Bacillus megaterium utilising the transpeptidase Sortase A from Staphylococcus aureus. We successfully constructed Mn-substituted BM3 and investigated its catalytic activity.

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