Maternal high salt diet altered Adenosine-mediated vasodilatation via PKA/BK channel pathway in offspring rats (vol 61, 1800390, 2017)

Scope: High salt (HS) diets are related to cardiovascular diseases, and prenatal HS was suggested to increase risks of coronary artery diseases in the offspring. This study tested the hypothesis that prenatalHSmay influence Adenosine-induced vasodilatation via protein kinase A (PKA) pathway in coronary arteries. Methods and results: Sprague-Dawley rats were fed with 8% salt diet for gestation, the control was fed with 0.3% salt diet. Coronary arteries from male adult offspring were tested for K+ channels and Adenosine signal pathways. Adenosine-mediated vasodilatation was reduced in coronary arteries in HS. There was no difference in gene expression ofA2A receptors between the two groups. After pretreatment with PKA inhibitor, vasodilatation to Adenosine was decreased to a smaller extent in HS than that in control. Forskolin (activator of adenylate cyclase)-mediated vasodilatation was decreased in HS. Iberiotoxin (large-conductance Ca2+-activated K+ channel [BK channel] inhibitor) attenuated Forskolin-induced vasodilatation in control, not inHSgroup. Currents of BK channels decreased in coronary artery smoothmuscle cells, and PKA-modulated BK channel functions were declined. Protein levels of BK beta 1 and PKA C-subunits in coronary arteries of HS offspring were reduced. Conclusions: Prenatal HS diets altered Adenosine-mediated coronary artery vasodilatation in the offspring, which was linked to downregulation of cAMP/PKA/BK channel pathway.