Generating a recombinant phosphothreonine-binding domain for a phosphopeptide of the human transcription factor, c-Myc.

•The FHA1 library yielded a reagent against a peptide from c-Myc.•The Myc-pTBD’s molecular determinants of specificity were identified.•The Myc-pTBD is comparable in specificity to commercially available antibodies.