From clinical sample to complete genome: Comparing methods for the extraction of HIV-1 RNA for high-throughput deep sequencing.

Marion Cornelissen,Astrid Gall,Monique Vink,Fokla Zorgdrager,Špela Binter,Stephanie Edwards,Suzanne Jurriaans,Margreet Bakker,Swee Hoe Ong,Luuk Gras,Ard van Sighem,Daniela Bezemer,Frank de Wolf,Peter Reiss,Paul Kellam,Ben Berkhout,Christophe Fraser,Antoinette C van der Kuyl

Virus Research（2017）

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摘要

•HIV-1 RNA was isolated from long-term stored serum or plasma samples.•QIAamp Viral RNA Mini Kit and manual isolation is superior compared to other methods.•Isolated viral RNA was used for genome amplification and deep sequencing.•Storage >25 years and low viral load decreased amplification of >3kb fragments.

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关键词

HIV-1,Complete genome,RNA isolation,QIAamp viral isolation kit,High-throughput deep sequencing

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