Flavobacterium zaozhuangense sp. nov., a new member of the family Flavobacteriaceae , isolated from metolachlor-contaminated soil

Strain ZZ-8 T , a Gram-negative, aerobic, non-spore-forming, non-motile, yellow-pigmented, rod-shaped bacterium, was isolated from metolachlor-contaminated soil in China. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain ZZ-8 T is a member of the genus Flavobacterium and shows high sequence similarity to Flavobacterium humicola UCM-46 T (97.2%) and Flavobacterium pedocola UCM-R36 T (97.1%), and lower (< 97%) sequence similarity to other known Flavobacterium species. Chemotaxonomic analysis revealed that strain ZZ-8 T possessed MK-6 as the major respiratory quinone; and iso-C 15:0 (28.5%), summed feature 9 (iso-C 17:1 w 9 c /C 16:0 10-methyl, 22.9%), iso-C 17:0 3-OH (17.0%), iso-C 15:0 3-OH (8.9%), iso-C 15:1 G (8.6%) and summed feature 3 (C 16:1 w 7 c /C 16:1 w 6 c , 5.7%) as the predominant fatty acids. The polar lipids of strain ZZ-8 T were determined to be lipids, a glycolipid, aminolipids and phosphatidylethanolamine. Strain ZZ-8 T showed low DNA–DNA relatedness with F. pedocola UCM-R36 T (43.23 ± 4.1%) and F. humicola UCM-46 T (29.17 ± 3.8%). The DNA G+C content was 43.3 mol%. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data and DNA–DNA hybridization, strain ZZ-8 T is considered a novel species of the genus Flavobacterium , for which the name Flavobacterium zaozhuangense sp. nov. (type strain ZZ-8 T = KCTC 62315 T = CCTCC AB 2017243 T ) is proposed.