Screening oligonucleotide sequences for silver staining and d-galactose visual detection using RCA silver staining in a tube.

Oligonucleotides were screened for strongly silver-stained repetitive sequences. An 'AG'-clustered purine sequence showed strong staining, and the staining density can be compromised by disrupting the continuity of the 'AG'-clustered sequence. The staining-favored sequence was then employed in rolling circle amplification (RCA) for its product detection. A tube-staining method was developed for convenient and visual RCA assay. Moreover, by introducing GalR into RCA, D-galactose was detected by RCA tube-staining with naked eyes without any equipment. About 10 mM D-galactose can be easily identified, and the detection of D-galactose was specific in comparison with that of several other monosaccharides.