Simple Method for Assembly of CRISPR Synergistic Activation Mediator gRNA expression array.

Johan Vad-Nielsen,Anders Lade Nielsen,Yonglun Luo

Journal of Biotechnology（2018）

引用 3|浏览2

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摘要

•A novel vector system for rapid generation of all-in-one CRISPR SAM gRNA expression array.•A simple and cost-effective system for generating a vector carrying up to 30 SAM gRNA expression blocks.•A useful system for CRISPR-mediated simultaneous activation of multiple genes.•Simultaneous activation of four EMT genes in human cells by CRISPR.

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关键词

CRISPR,CRISPRa,SAM,Golden gate assembly,Gene activation

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