Development of a tetra-primer ARMS-PCR for detecting the E198A SNP in the isotype-1 β-tubulin gene of Haemonchus contortus populations in China.

Zhang Zongze,Yang Xin,Ali Ahmad Awais,Lei Weiqiang,Wang Chunqun,Wenda Di,Zhou Yanqin,Zhao Junlong,Fang Rui,Hu Min

Veterinary Parasitology（2018）

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摘要

•A Tetra-primer ARMS-PCR was developed to detect E198A in H. contortus.•This new method is specific and sensitive enough to perform in application.•The accuracy is also very high.

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关键词

Haemonchus contortus,Tetra-primer ARMS-PCR,Benzimidazole resistance,SNP

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