Efficient evaluation of humoral immune responses by the use of serum pools.

•A reliable and rapid method to confirm or reject hypotheses enables more efficient research.•A fast evaluation of humoral immune responses using serum pools of only 15μL/sample.•Pool-testing validated in different assays: ELISA, line blot, IF microscopy.•EBV antibodies and autoantibodies in systemic autoimmune diseases.•Results between applied assays and literature are consistent.