Efficient evaluation of humoral immune responses by the use of serum pools.

Louise Sternbæk,Anette H Draborg,Christoffer T Nielsen,Søren Jacobsen,Line V Iversen,Lone Troelsen,Elke Theander,Gunnar Houen

Journal of Immunological Methods（2017）

引用 4|浏览8

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摘要

•A reliable and rapid method to confirm or reject hypotheses enables more efficient research.•A fast evaluation of humoral immune responses using serum pools of only 15μL/sample.•Pool-testing validated in different assays: ELISA, line blot, IF microscopy.•EBV antibodies and autoantibodies in systemic autoimmune diseases.•Results between applied assays and literature are consistent.

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关键词

Serum pools,ELISA,Line blot,Screening,Serology

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