Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells

Plansky Hoang,Jason Wang,Bruce R Conklin,Kevin E Healy,Zhen Ma

NATURE PROTOCOLS（2018）

引用 117|浏览20

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摘要

This protocol describes the generation of early-developing cardiac organoids from human pluripotent stem cells. Geometric confinement of the hiPSCs drives spatial organization of the cells from a 2D layer into 3D cardiac microchambers.

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关键词

Cell lineage,Heart development,Imaging,Organogenesis,Stem-cell differentiation,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry

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