[Whole glucan particle promotes the maturation of tumor-educated mouse dendritic cells to suppress regulatory T cell differentiation].

Objective To investigate the maturation and immune responses of tumor-educated dendritic cells (TEDCs) stimulated by whole glucan particle (WGP). Methods Mouse bone marrow-derived dendritic cells were generated by Flt3 ligand and co-cultured with interleukin 10 (IL-10) and transforming growth factor β (TGF-β) in vitro to induce TEDCs, and then stimulated by WGP for 2 days. CD4+ FOXP3+ T cells from the lymph nodes and spleens of OT-II mice were purified by magnetic-activated cell sorting, and then co-cultured with TEDCs in the presence of ovalbumin (OVA). Expressions of cell surface markers (CD11c, CD86, CD40, CD80, MHC-II) and CD4+ FOXP3+T cell differentiation and proliferation were detected by flow cytometry. real-time quantitative PCR was used to detect the mRNA levels of TNF-α, IL-12p40, IL-10, IL-6, IL-4, TGF-β1 and ELISA was used to detect the levels of TNF-α, IL-23, IL-12p70, IL-4 in culture supernatant. Results WGP up-regulated MHC-II and co-stimulation molecules CD86, CD80, CD40 on TEDCs, increased the levels of TNF-α, IL-12p40, IL-6 in TEDCs, decreased the production of TGF-β1. Furthermore, WGP also suppressed CD4+FOXP3+ T cell differentiation and proliferation in vitro. Conclusion WGP improves TEDC maturation and inhibits immunosuppressive functions.