An easily transfectable cell line that produces an infectious reporter virus for routine and robust quantitation of Kaposi's sarcoma-associated herpesvirus reactivation.

•Vero.rKSHV.294 cells are easily transfectable.•Vero rKSHV.294 cells provide a robust and reliable system to quantitate production of infectious KSHV in response to ectopic proteins.•Ectopic Rta, but not NICD-1, is sufficient to reactivate KSHV from latency.