A replication-competent foot-and-mouth disease virus expressing a luciferase reporter.

•We have generated a replication-competent foot-and-mouth disease virus expressing Nanoluciferase, designated as Nano-FMDV.•Nano-FMDV is genetically stable.•The replication of Nano-FMDV can be monitored by bioluminescent methods.•This reporter virus has potential applications in real-time monitoring of FMDV infection in vitro and in vivo, and in screening of antivirals and antibodies.