A Semi-automated High-Throughput Microtitration Assay for Filoviruses.
Methods in molecular biology (Clifton, N.J.)(2017)
摘要
The 50% tissue culture infectious dose (TCID) endpoint dilution assay is one of the gold standard methods for measuring filovirus infectivity. We have increased virology microtitration assay throughput at biosafety level (BSL)-4 by implementing automated liquid handling and semi-automated assay endpoint readout. Utilization of automated liquid handling for cell plating and virus dilution along with optimization of the assay endpoint readout, using a luminescent-based cell viability assay and an automated plate reader, has improved workflow efficiency, reduced operator burden and assay time, decreased assay variability, and increased data return.
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关键词
Assay,Biosafety level 4 (BSL-4),Dilution,Ebola,Efficiency,Endpoint,Filovirus,High-throughput screening (HTS),Liquid handling,Luminescence,Luminescent,Marburg,Microtitration,Semi-automated,Tissue culture infectious dose (TCID50),Viability
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