Metabolic labeling and recovery of nascent RNA to accurately quantify mRNA stability.
Methods(2017)
摘要
•Accurate assessment of RNA stability is key to characterizing gene expression.•4-Thiouridine labeling has major advantages over methods that inhibit transcription.•Quality control and normalization are essential to generate reproducible half-lives.•The relative amount of 4sU-labeled nascent RNA influences accurate quantification.
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关键词
4sU,DRB 5,iPSCs
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