Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion.
MICROCHIP DIAGNOSTICS: METHODS AND PROTOCOLS(2017)
摘要
We describe a droplet microfluidics method to screen for multiple mutations of a same oncogene in a single experiment using passive droplet fusion. Genomic DNA from H1573 cell-line was screened for the presence of the six common mutations of the KRAS oncogene as well as wild-type sequences with a detection efficiency of 98 %. Furthermore, the mutant allelic fraction of the cell-line was also assessed correctly showing that the technique is quantitative.
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关键词
Biomarkers,Cancer,Digital PCR,Droplet microfluidics,KRAS,Multiplexing,Passive droplet fusion
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