Proteome-wide identification of ubiquitin interactions using UbIA-MS

Xiaofei Zhang,Arne H Smits,Gabrielle BA van Tilburg,Huib Ovaa,Wolfgang Huber,Michiel Vermeulen

NATURE PROTOCOLS（2018）

引用 353|浏览23

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摘要

This protocol describes the chemical synthesis of different nonhydrolyzable diubiquitin linkages and their use in affinity purification and LC–MS/MS identification of linkage-selective diubiquitin interactors.

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关键词

Mass spectrometry,Protein–protein interaction networks,Proteomics,Solid-phase synthesis,Ubiquitins,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry

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