Expression and Purification of SH2 Domains Using Baculovirus Expression System.
Methods in molecular biology (Clifton, N.J.)(2017)
摘要
Recombinant proteins expressed in bacteria are sometimes insoluble, aggregated, and incorrectly folded. For those Src homology 2 (SH2) domains that are insoluble in bacteria, baculovirus-insect cell expression systems can be an alternative to produce soluble and functionally active proteins. We describe a protocol for cloning and purification of GST-tagged SH2 domains using the Bac-to-Bac baculovirus expression system.
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关键词
Bac-to-Bac,Baculovirus expression system,GST fusion protein,Insect cells,SH2 domain
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