Real-Time Monitoring Of The Aggregation Of Alzheimer'S Amyloid-Beta Via H-1 Magic Angle Spinning Nmr Spectroscopy

Proton magic-angle-spinning NMR used for real-time analysis of amyloid aggregation reveals that mechanical rotation of A beta(1-40) monomers increases the rate of formation of aggregates, and that the increasing lag-time with peptide concentration suggests the formation of growth-incompetent species. EGCG's ability to shift off-pathway aggregation is also demonstrated.