Validation of water-borne steroid hormones in a tropical frog (Physalaemus pustulosus).

General and Comparative Endocrinology(2018)

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摘要
•We quantified excreted CORT, estradiol and progesterone in water in tungara frogs.•We used challenges, dose response, ELISA and HPLC-MS to measure three steroids.•A one-hour water bath captures physiologically informative hormone concentrations.•Water-borne CORT and estradiol were correlated with plasma and homogenate levels.
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关键词
Corticosterone,Estradiol,Non-invasive endocrinology,Progesterone,Stress,Water-borne hormones
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