Evaluation of five automated and one manual methods for Toxoplasma and human DNA extraction from artificially spiked amniotic fluid.

Hélène Yera, Louise Ménégaut,Marie-Pierre Brenier-Pinchart,Feriel Touafek,Patrick Bastien,Frédéric Dalle

Clinical Microbiology and Infection（2018）

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摘要

Despite the influence of the subsequent PCR method used, this study should help microbiologists in the choice of DNA extraction methods for the detection of T. gondii in amniotic fluid. The extraction method should be checked as adequate to the PCR assay used.

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关键词

Congenital toxoplasmosis,Diagnosis,DNA extraction,Polymerase chain reaction,rep529,Toxoplasma gondii

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