High degree of correlation between Ebola virus BSL-4 neutralization assays and pseudotyped VSV BSL-2 fluorescence reduction neutralization test.

Krishnamurthy Konduru,Amy C Shurtleff,Sina Bavari,Gerardo Kaplan

Journal of Virological Methods（2018）

引用 20|浏览12

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摘要

•Neutralization assay based on VSV construct containing the EBOV GP and the GFP gene.•GFP is expressed in infected cells.•EBOV neutralizing antibodies reduce GFP fluorescence in a dose-dependent manner.•BSL2 Fluorescence Reduction Neutralization Test (FRNT) yield results in 26 h.•High degree correlation of BSL2 FRNT and BSL4 Plaque Reduction Neutralization Test (PRNT).

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关键词

Filoviridae,Immune response,Neutralizing antibodies,Replication-competent recombinant vesicular stomatitis virus,Glycoprotein,Green fluorescence protein

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