Culture-Independent Detection of Nontuberculous Mycobacteria in Clinical Respiratory Samples.

Gianny P Scoleri,Jocelyn M Choo,Lex E X Leong,Thomas R Goddard,Lisa Shephard,Lucy D Burr,Ivan Bastian,Rachel M Thomson,Geraint B Rogers

JOURNAL OF CLINICAL MICROBIOLOGY（2016）

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摘要

Culture-based detection of nontuberculous Mycobacteria (NTM) in respiratory samples is time consuming and can be subject to overgrowth by nonmycobacterial bacteria. We describe a single-reaction TaqMan quantitative PCR assay for the direct detection of NTM species in clinical samples that is specific, sensitive, and robust.

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