The Physcomitrella patens System for Transient Gene Expression Assays.
PLANT SYNTHETIC PROMOTERS: METHODS AND PROTOCOLS(2016)
摘要
Transient expression assays are valuable techniques to study in vivo the transcriptional regulation of gene expression. These methods allow to assess the transcriptional properties of a given transcription factor (TF) or a complex of regulatory proteins against specific DNA motifs, called cis-regulatory elements. Here, we describe a fast, efficient, and reliable method based on the use of Physcomitrella patens protoplasts that allows the study of gene expression in a qualitative and quantitative manner by combining the advantage of GFP (green fluorescent protein) as a marker of promoter activity with flow cytometry for accurate measurement of fluorescence in individual cells.
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关键词
Physcomitrella patens,Protoplasts,Subcellular localization,Transcription factor,Transient expression
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