Discovery of enzymatically depolymerized heparins capable of treating Bleomycin-induced pulmonary injury and fibrosis in mice

•A combined heparinases depolymerized heparin libraries were built and systematically screened for the anti-pulmonary injury and fibrosis efficacy directly with Bleomycin challenged mice model.•Structural properties of the efficient components have been characterized by combination use of chip-based amide hydrophilic interaction chromatography HILIC- FT-ESI–MS, PAGE, and High performance liquid chromatography (HPLC).•The possible mechanisms that heparins exert their effects on have been primarily evaluated.