Detection of Bacterial Quorum Sensing Molecules.

HOST-PATHOGEN INTERACTIONS: METHODS AND PROTOCOLS(2018)

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摘要
Bacterial cells use the quorum sensing system to communicate with each other. The gram-negative species very often use N-acyl homoserine lactones for this purpose. One of the easiest ways to detect these molecules is the use of particular reporter strains, which possess different kinds of reporter genes under the control of AHL-responsive promoters. Here we present some of the possibilities available today, even for not specialized researchers.
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关键词
Acyl homoserine lactones,GFP,HPLC-MS,Image processing,Quorum sensing
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