Optimal DNA Isolation Method for Detection of Nontuberculous Mycobacteria by Polymerase Chain Reaction.

Samira Mohammadi,Bahram Nasr Esfahani,Sharareh Moghim,Hossein Mirhendi,Fatemeh Riyahi Zaniani,Hajieh Ghasemian Safaei,Hossein Fazeli,Mahshid Salehi

Advanced biomedical research（2017）

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摘要

According to the electrophoresis results, the CTAB and Chelex DNA extraction methods were more successful in comparison with the others as regard producing suitable concentrations of DNA with the minimum use of PCR inhibitor.

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关键词

DNA extraction methods,heat-shock protein 65,nontuberculous mycobacteria,polymerase chain reaction

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