Metabolism of the synthetic cannabinoid 5F-PY-PICA by human and rat hepatocytes and identification of biliary analytical targets by directional efflux in sandwich-cultured rat hepatocytes using UHPLC-HR-MS/MS.

•24 metabolites of 5F-PY-PICA identified by LC-HR-MS/MS after hepatocytes incubation.•Analytical targets identified with directional efflux from 3 d cultured SCRH.•Two metabolites proposed as biliary analytical targets.•Results help further understand overall disposition of synthetic cannabinoids.