Hormonal profiling: Development of a simple method to extract and quantify phytohormones in complex matrices by UHPLC-MS/MS.

•Development of a fast and simple method for plant growth regulators analysis.•Avoids the use of tedious and highly time consuming steps.•Feasible to obtain 20 different compounds in only one round of extraction.•Capable of quantify in one injection all analytes extracted.•Optimized in complex plant matrices.