Identification of essential and non-essential genes in Ambystoma tigrinum virus.

Mariah M Aron,Alexander G Allen, Mathew Kromer,Hector Galvez,Brianna Vigil,James K Jancovich

Virus Research（2016）

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摘要

•Develop a standardized protocol and procedure to generate recombination cassettes used to knock-out ranavirus genes.•Optimized the recombination protocol to generate a knock-out ranavirus.•Identified 2 essential, one semi-essential and 2 non-essential genes in Ambystoma tigrinum virus.

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关键词

Ranavirus,Homologous recombination,Gene knock-out,Viral replication pathogenesis

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