Peptidome Analysis of Transudative Pleural Effusion by Ultra-filtration Coupled with Nano-Liquid Chromatography-Tandem Mass Spectrometry

The peptides, proteins and other biological molecules in transudative pleural effusion correlate directly or indirectly with specific physiological and pathological state, reflecting the information regarding the lungs or other parts of the body. In the present study, the peptide fraction in transudative pleural effusion was isolated by ultrafiltration. After desalted and enriched by C-18 tips, the peptide mixture was analyzed by nano LC. MS/MS. The results showed that 314 peptides, which were originated from 52 proteins, in pleural transudate were identified. More than half of the peptides were derived from fibrinogen. Many peptides were characterized as displaying ladder sequences. In addition, a large number of proline oxidation modifications were detected in the peptides derived from collagen and fibrinogen. Gene ontology enrichment analysis showed that the most of the proteins extracellular properties of pleural transudate polypeptide components were protein with exocytosis. The study provided a rapid and efficient separation and analysis methods for lung disease method for screening peptide biomarkers related to lung diseases from transudative pleural effusion.