A Radial Microfluidic Array For Studying Single-Cell Ca2+ Dynamics Stimulated By Dynamic Atp Signals

Miao Yu,Zong-Zheng Chen,De-Pei Zeng,Xing-Han Li,Bo Liu,Cheng Xiang,Kai-Rong Qin

2018 IEEE 14TH INTERNATIONAL CONFERENCE ON CONTROL AND AUTOMATION (ICCA)（2018）

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摘要

A radial microfluidic array is proposed to analyze intracellular calcium dynamics in response to dynamic extra-cellular adenosine triphosphate (ATP) stimulation at single cell level. The microfluidic array consists of multiple single cell trapping units in radial distribution with same angle, each unit is designed by a combination of stagnation point flow and physical barrier. Cellular experimental results verified that the effectiveness of the single cell trapping and intracellular calcium dynamic behaviors in response to static or dynamic extra-cellular ATP stimulation.

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关键词

single-cell Ca2+ dynamics,multiple single cell trapping units,stagnation point flow,dynamic extracellular ATP stimulation,static ATP stimulation,intracellular calcium dynamic behaviors,radial distribution,multiple single cell,single cell level,dynamic extracellular adenosine triphosphate stimulation,intracellular calcium dynamics,dynamic ATP signals,radial microfluidic array,Ca2+

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