Spirosoma gilvum sp. nov., Isolated from Beach Soil

A Gram-reaction-negative, nonmotile, rod-shaped, aerobic bacterial strain, designated 15J10-9T5 T , was isolated from beach soil on Jeju Island, South Korea, and was characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that the isolate belonged to the family Cytophagaceae in the phylum Bacteroidetes , and was most closely related to Spirosoma aerolatum PR1012k T (96.1%) and Spirosoma panaciterrae DSM 21099 T (95.7%). The detection of menaquinone MK-7 as the predominant respiratory quinone, a fatty acid profile with summed feature 3 (C 16:1 ω 7 c /C 16:1 ω 6 c ), C 16:1 ω 5 c , and iso-C 15:0 as the major components, and a polar lipid profile with phosphatidylethanolamine as the major component also supported the affiliation of strain 15J10-9T5 T in the genus Spirosoma . The G+C content of the genomic DNA was 47.7 mol %. The isolate could be clearly differentiated from its closest neighbors based on its phenotypic, phylogenetic, chemotaxonomic, and genotypic features. Therefore, strain 15J10-9T5 T represents a novel species of the genus Spirosoma , for which the name Spirosoma gilvum sp. nov. is proposed. The type strain is 15J10-9T5 T (=KCTC 52033 T = JCM 31997 T ).