Identification of a Nitroalkane Oxidase Gene: Related to the Growth of

Current Microbiology(2008)

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摘要
encoding a nitroalkane oxidase that can catalyze toxic nitroalkanes to their corresponding aldehydes or ketones and hydrogen peroxide, was cloned from , but its function related to the growth of is unknown. was disrupted by the insertion of a kanamycin-resistance gene; the resulting strain can grow earlier than a wild-type strain under the same conditions. It was shown that disruption accelerated growth of the disruption mutant, which could restore its phenotype and morphology as a wild-type strain by complementation of a single copy number of inserted into the chromosome. The introduction of an extra copy of into the wild-type strain resulted in delayed growth. The result suggested that is an important gene related to the growth of .
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关键词
Streptomyces,Extra Copy,Conjugal Transfer,Apramycin,Nikkomycin
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