Identification and mapping of ten new potential insulators in the region of human chromosome 19q13.12

A positive-negative selection system revealed 10 potential insulators able to block enhancer interaction with promoter in the 106 bp human chromosome 19 region between genes FXYD5 and COX7A1. Relative positions of insulators and genes are in accord with the hypothesis that insulators subdivide genomic DNA into independently regulated loop domains.