sp. nov., isolated from wild in northwestern China

A Gram-stain-negative strain, 23-3-2, was isolated from a nodule of grown in Northwest China. Phylogenetic analysis of 16S rRNA gene sequence showed that the strain was closely related to CCNWXJ 40-4 and CCNWXJ 12-2 having 98.0 and 97.9% similarities, respectively. Phylogenetic analysis based on the protein-coding genes and showed lower similarity with the same closely related species (94.5 and 89.9%, respectively), which suggest that 23-3-2 strain represents a distinctly delineated genospecies of the genus . The 23-3-2 strain grew at 20–37 °C temperature (optimum 28 °C) and 5.0–9.0 pH range (optimum pH 7.0). The cells contained Q-10 as the sole respiratory quinone and 18:1ω7 (24.56%) as the major cellular fatty acid. The DNA relatedness between the strain 23-3-2 and the two reference strains was 39–44%. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain 23-3-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 23-3-2 (= CGMCC 1.15528 = NBRC 112337). The respective DPD Taxon Number is TA00147.