Calcium-dependent interaction of monomeric S100P protein with serum albumin

•Monomeric human S100P protein interacts with HSA in excess of Ca2+ with Kd of 25–50nM.•Calcium removal or S100P dimerization abolish the S100P-HSA interaction.•S100P-HSA interaction is selective.•in vitro glycation of HSA disables its binding to S100P.•Functional properties of monomeric and dimeric forms of S100 proteins are different.