The Efficacy Of Rgd Modified Liposomes Loaded With Vinorelbine Plus Tetrandrine In Treating Resistant Brain Glioma

Brain glioma is one of the most common and devastating intracranial malignancies with a high mortality. Chemotherapy for brain glioma is not ideal due to blood brain barrier (BBB) and multidrug resistance (MDR). The objectives of the present study were to develop a kind of RGD (Arg-Gly-Asp) tripeptide modified vinorelbine plus tetrandrine liposomes to achieve BBB transportation, MDR reversion and glioma cell targeting simultaneously. The studies were performed on glioma cells, resistant glioma cells and glioma-bearing mice. Results showed that the constructed liposomes with suitable physicochemical properties could significantly enhance the transport across BBB, obviously accumulate in glioma cells, and exhibit evident capabilities in diminishing brain glioma in mice. Action mechanism studies indicated that the enhanced anticancer efficacy could be attribute to the follows: prolonged elimination half-life (7.093 +/- 1.311h); increased AUC(0-24h) (28.92 +/- 2.66mg/L*h); transporting across BBB; enhanced cellular uptake; down-regulation on P-gp (0.49 +/- 0.06 fold); inducing apoptosis via activating caspase 8, 9, and 3 (2.40 +/- 0.22, 3.57 +/- 0.29, and 4.33 +/- 0.30 folds, respectively). In conclusion, the RGD modified vinorelbine plus tetrandrine liposomes may offer a promising therapeutic strategy for treatment of brain glioma.