Generation of conditional Acvrl1 knockout mice by CRISPR/Cas9-mediated gene targeting.

•This study is to generate mutant mice containing the Alk1 gene flanked with LoxP sequences to allow the conditional deletion of Alk1 by the LoxP/Cre system.•CRISPR/Cas9 technique was used to edit Alk1.•CRISPR/Cas9 technique was able to efficiently modify Alk1 and create the target mice.