Comprehensive phospholipid and sphingomyelin profiling of different brain regions in mouse model of anxiety disorder using online two-dimensional (HILIC/RP)-LC/MS method.

•Development of novel online 2D-LC/MS method for comprehensive analysis of phospholipids and sphingomyelins in brain sample.•Profiling of PL and SM composition of DHPC, VHPC and PFC brain regions was achieved in mouse model of anxiety disorder.•In case of 37 PL and SM species, significant differences were observed between AX and nAX mice.