Disulfide bond formation protects Arabidopsis thaliana glutathione transferase tau 23 from oxidative damage.

Maria-Armineh Tossounian,Inge Van Molle,Khadija Wahni,Silke Jacques,Kris Gevaert,Frank Van Breusegem,Didier Vertommen,David Young,Leonardo Astolfi Rosado,Joris Messens

Biochimica et Biophysica Acta (BBA) - General Subjects（2018）

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摘要

•GSTU23 methionine oxidation is the major oxidative modification at lower H2O2 concentration.•A disulfide bond protects active site cysteines and methionines from overoxidation.•Oxidized GSTU23 is redox regulated by methionine sulfoxide reductases and glutaredoxin.•To compensate the drop in kcat at a higher H2O2 concentration, a favourable enzyme:substrate complex is formed.•X-ray structures of oxidized GSTU23 show methionine oxidation and local unfolding around Cys110.

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关键词

Glutathione transferase,Kinetics,X-ray structure,Thermodynamics,Methionine sulfoxide,Disulfide bond

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