Transfection in Primary Cultured Neuronal Cells.
Methods in molecular biology (Clifton, N.J.)(2017)
摘要
Transfection allows the introduction of foreign nucleic acid into eukaryotic cells. It is an important tool in understanding the roles of NMDARs in neurons. Here, we describe using lipofection-mediated transfection to introduce cDNA encoding NMDAR subunits into postmitotic rodent primary cortical neurons maintained in culture.
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关键词
Culture,Lipofectamine®,Lipofection,NMDA,Neuron,Transfection
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